Effect of streptovitacin A on replication of an RNA virus (poliovirus).

Abstract

SummaryStreptovitacin A, a highly efficient inhibitor of protein synthesis, at 10 μg/ml of medium completely prevented synthesis of infectious poliovirus (type 1) and of CF antigen, as well as of CPE, in BS-C-1 cells when it was added up to about an hour before the end of the eclipse phase and was then left in the medium for the duration of the experiment. When the drug was added at the very end of the eclipse period, before new infectious virus had appeared, and was then left in the medium, a small amount of new virus appeared in the cells. When the drug was added during the exponential phase of viral multiplication, there was always some further increase in infectious virus but never at the level achieved in the untreated cultures. When the drug was added one or two hours after infection and washed away one or two hours later, the subsequent delay in viral synthesis was always considerably longer than the time the drug was present in the culture medium. These results fit the assumption that protein synt...