Abstract
BackgroundStreptococcus anginosus (S. anginosus) was reported increased in oral squamous cell carcinoma (OSCC) tissue. The aim of this study was to investigate the response of oral cancer cells in the biological characteristics evoked by the S. anginosus and investigate its potential mechanisms.MethodsThe growth curve and concentration standard curve of S. anginosus were determined, and a series of concentrations of S. anginosus supernatant were applied to OSCC cell lines SCC15, then selected an optimal time and concentration by CCK-8 assay. Then autophagic response, proliferative activity, cell cycle and apoptosis, invasion and migration abilities were evaluated in SCC15.ResultsThe results showed that when the ratio of S. anginosus supernatant to cell culture medium was 1:1 and the co-culture time was 16 h, the inhibitory effect on SCC15 was the most obvious; Furthermore, the supernatant of Streptococcus upregulated the autophagy activity of SCC15, thus significantly inhibiting its proliferation, migration and invasion ability. Compared with control groups, the cell cycle showed G1 arrest, S and G2/M phases decreased, and the percentage of apoptotic cells relatively increased (P < 0.05).ConclusionS. anginosus reduced the proliferation, migration and invasion of SCC15 cells and promoted cell apoptosis; Moreover, autophagy may be one of the mechanisms in this process.
Highlights
Streptococcus anginosus (S. anginosus) was reported increased in oral squamous cell carcinoma (OSCC) tissue
Real time PCR analysis Total RNA was isolated from SCC15 cells using Trizol method (Beyotime, China). cDNA was synthesized by qScript cDNA synthesis kit (Sigma, USA) and primers synthesized by Wuhan Sevier Biotechnology Co., Ltd
The stable phase 20 h bacterial solution was selected for experiment because the amount of primary and secondary metabolites of bacteria will reach the maximum in this period
Summary
Streptococcus anginosus (S. anginosus) was reported increased in oral squamous cell carcinoma (OSCC) tissue. In the 1990s, the researchers found that there may be a relationship existed between microbes and tumors [1]. With the development of technologies like metagenomics and high-throughput sequencing, research on microbes and human health and diseases has gradually started. More and more studies have found that in some digestive tract tumors, the composition of the flora has changed significantly, and some bacteria have increased or decreased in specificity. Helicobacter pylori has been identified as an independent risk factor for gastric cancer, while studies have reported that beneficial bacteria such as bifidobacteria can inhibit the occurrence and development in gastric tumors. The metabolism of cancer cells will become a promising therapeutic target, and the role of microorganisms in cancer treatment is becoming more and more important [6]
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