Effect of Straw and Straw Biochar on the Community Structure and Diversity of Ammonia-oxidizing Bacteria and Archaea in Rice-wheat Rotation Ecosystems

Hanlin Zhang,Juanqin Zhang,Shuangxi Li,Huifeng Sun,Xianqing Zheng,Weiguang Lv,Sheng Zhou,Naling Bai

doi:10.1038/s41598-019-45877-7

Hanlin Zhang, Juanqin Zhang + Show 6 more

Open Access

https://doi.org/10.1038/s41598-019-45877-7

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Abstract

Ammonia oxidation is the first and rate-limiting step of nitrification, driven by ammonia-oxidizing bacteria (AOB) and ammonia-oxidizing archaea (AOA). Straw and straw biochar retention are the popular ways to utilize the agricultural by-products in China, but their long-term effects on AOB and AOA still remain poorly understood. Based on a 7-year plot experiment, which had 4 fertilization regimes: no fertilizer (CK), regular fertilization (RT), straw retention (SR) and straw biochar retention (SB), the abundance and the composition of AOB and AOA was investigated before both the harvest of rice and wheat season by quantitative PCR and 454 high-throughput pyrosequencing, respectively. (1) Compared to RT, straw and straw biochar increased AOB abundance and diversity significantly only in wheat season (P < 0.05), and they both ranked as SB > SR > RT. Among fertilized treatments, a significant difference between SR and RT was found in AOB community composition of the winter season (R value = 0.58, P value = 0.02); (2) In contrast, AOA was almost not responsive to organic addition, except the significant enhancement of abundance by biochar in wheat season; (3) After straw and straw biochar addition, soil potential nitrification rates (PNR) was positive correlated to AOB abundance in both rice and wheat season (P < 0.01), not to AOA abundance (P = 0.211 and 0.068, respectively). This study provides scientific support for the potential of straw utilization to improve nitrification in rice-wheat rotation system with respect to soil ammonia oxidation microorganism.

Highlights

Soil pH was commonly considered as a key driving environmental factor influencing ammonia-oxidizing bacteria (AOB) and ammonia-oxidizing archaea (AOA) community
There was no significant difference in all measured properties between straw retention (SR) and straw biochar retention (SB)
Our study showed that compared to regular fertilization (RT) and SR, SB stimulated the growth of AOA and AOB in the wheat season, but there was no significant difference among the fertilization treatments in the rice season

Summary

Introduction

Soil pH was commonly considered as a key driving environmental factor influencing AOB and AOA community. In addition to soil properties, both short-terms and long-terms chemical fertilization can increase AOB abundance and alter its community composition, but almost have no impact on AOA11,12 Organic inputs such as organic fertilizer, straw, biochar affect AOB community significantly, but maybe because of the mixtrophic character of AOA, have www.nature.com/scientificreports/. The aims of this research were under the effects of straw and straw biochar (1) to identify the abundance and community composition shifts of AOA and AOB in rice and wheat season; (2) to detect the relative contribution of AOA and AOB to nitrification seasonally; (3) to provide scientific support for the establishment of a straw utilization technique to efficiently conserve the diversities of AOB and AOA communities

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