Effect of strains and extraction methods on β-glucan production, antioxidant properties, and FTIR Spectra from mushroom fruiting bodies of Schizophyllum commune Fr. in Thailand

N Mongkontanawat,D Thumrongchote

doi:10.26656/fr.2017.5(4).259

Abstract

Schizophyllum commune Fr., a native mushroom of Thailand, has a high nutritional value and is classified as a mushroom with medicinal properties that can neutralize the growth of many cancer cells. This research aimed to study the effect of S. commune strains and the extraction methods on the quantity and properties of β-glucan. The five S. commune Fr. strains used in this research consisted of Chanthaburi, 85-022, 85-023, 85-031, and 85- 043. There were two different β-glucan extraction methods employed: hot water (M1) and hot alkali extraction (M2), which were compared with the control (native-MR). The results indicated that the Chanthaburi strain has the highest β-glucan content 49.20±0.35% (w/w), and high potential antioxidant activity (79.14±0.77 DPPH% and 50.92±0.48 ABTS%) (p < 0.05). The extraction methods did not affect the yield of β-glucan, except the antioxidant properties and chemical structure of the extract substance. The extract substance from M2 has significantly the highest potential antioxidant activity (80.22±0.51). A mushroom juice drink in cans was developed using 1-day-old MR and adjusted pH of more than 7, which can increase the antioxidant properties of the product.

Highlights

Schizophyllum commune Fr., an edible mushroom, is a native mushroom that grows on logs in Thailand’s forests
It was reported that S. commune (MCCT 38) in Tripura, India consisted of 15.55% crude protein, 42% total carbohydrates and 30.0% crude fibre (Debnath et al, 2017), which differs from the S. commune (MCCT 38) in Nagaland, India that consisted of 22.50% crude protein, 32.43% total carbohydrates and
In this research, 1-dayold S. commune Fr. mushroom fruiting bodies dried powder from five strains was selected: Chanthaburi, 85-022, 85-023, 85-031, and 85-043, which are found in Thailand, for β-glucan production

Summary

Introduction

Schizophyllum commune Fr., an edible mushroom, is a native mushroom that grows on logs in Thailand’s forests. Schizophyllum commune Fr. contains Schizophyllan (β-1, 3-glucan), an anticancer substance (Lemieszek and Rzeski, 2012), and antioxidant compounds that have anti-ageing properties (Pirshahid et al, 2011). The chemical composition of substrate produced from S. commune influenced βglucan content, phenolic content, and antioxidant activity (Klaus et al, 2011; Basso et al, 2020). Schizophyllum commune is grown in different regions and has various chemical compositions. It was reported that S. commune (MCCT 38) in Tripura, India consisted of 15.55% crude protein, 42% total carbohydrates and 30.0% crude fibre (Debnath et al, 2017), which differs from the S. commune (MCCT 38) in Nagaland, India that consisted of 22.50% crude protein, 32.43% total carbohydrates and

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