Effect of storage time on metabolic function of apheresis platelets

Abstract

Objective To study the effect of storage time on metabolic function of apheresis platelets. Methods Selected a total of 30 bags of apheresis platelets which were collected from 30 cases of voluntary blood donors as research subjects. The volume of each bag of apheresis platelets was one therapeutic dose, and platelet count was (2.5~3.0)×1011/L. All of 30 bags of apheresis platelets would been stored in storage tank for 5 days at 22 ℃. The 30 bags of apheresis platelets were randomly divided into storage for 1 day group (n=10), storage for 3 days group and storage for 5 days group (n=10) equally by digital table method after being numbered. Content of lactic acid, lactate dehydrogenase (LDH), content of glucose and pH value were detected among 3 groups. Relationship of the metabolic function of apheresis platelets and storage time were analyzed by statistical method. Results The longer the storage duration time of apheresis platelets was, the lower of pH values and content of glucose in apheresis platelets were, and there were significant differences among 3 groups (P<0.05). At the same time, contents of LDH and lactic acid in apheresis platelets were gradually increased, and there also were significant differences among 3 groups (P<0.05). Conclusions There are marked changes for metabolic function of apheresis platelets in storage of 5 days at 22 ℃. The shorter the storage duration time of apheresis platelets is, the better quality of apheresis platelets is. Key words: Blood component transfusion; Platelets transfusion; Apheresis platelets; Metabolism; Platelet function tests; Blood preservation; Clinical blood transfusion