Abstract

Introduction: Storing teeth in media other than water decreases bacterial, viral and fungal growth and prevents enamel desiccation and allows teeth to be stored before testing. Materials and methods: One hundred and eighty freshly extracted human premolars for orthodontic purpose were chosen for the study. The samples were divided into six groups of 30 teeth each. Each group was stored in the following media for 2 months Group I-distilled water, Group II-10% formalin, Group III-saline solution, Group IV-70% ethanol, Group V-3% hydrogen peroxide, Group VI-artificial saliva. Results: The measurements were computed statistically. The result showed that Group II (10% formalin) had highest mean shear bond strength of 9.93 ± 0.858 MPa, Group IV (70% ethanol) had lowest mean shear bond strength of 3.63 ± 0.834 MPa. Conclusion: Shear bond strength with isotonic saline solution and distilled water were 7.59 and 6.15 MPa respectively which was comparable to clinically acceptable bond strength of 6 to 8 MPa; hence can be safely recommended as the most effective storage media.

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