Effect of Steroidal Saponins and Flavonoid from <i>Fructus tribuli</i> on Growth of <i>Lactobacillus </i><i>brevis</i>

Abstract

Effect of steroidal saponins and flavonoid from Fructus tribuli on growth of Lactobacillus brevis was studied by measuring the optical density at 600nm and pH using De Man, Rogosa and Sharpe (MRS) broth as the control. The addition (mg/ml) of steroidal saponins was 0.10, 0.20, 0.30, 0.40 and 0.50, and the addition (mg/ml) of flavonoid was 0.10, 0.15, 0.20, 0.25 and 0.30. Results were as follows: The additions of steroidal saponins and flavonoid had a significant promotion on the growth of Lactobacillus brevis. The optimum concentrations of steroidal saponins and flavonoid were 0.20mg/ml and 0.15mg/ml respectively.