Effect of Sterilization methods and Replication in the Microbial community and the Growth of the Soybean plant Glycine max L. in the Gypsum soil

Abstract

Pots experiment was conducted to study the effect of sterilization methods and resterilization on the microbial community and numbers and the growth of soybean plant characteristics, The results indicated that the effect of sterilization methods and replicated were significant on the number of bacteria and fungi , and on growth characteristics of soybean plant , the number of bacteria and fungi were decrease significantly before and after the sowing by the effect of Autoclave and formalin sterilization in compare with non sterilized treatment , the two time of sterilization cause additional significant decreas , the lowest number recorded at replicated formalin treatment ,The percentage of mycorrhizal infection was decrease significantly by sterilization , the replicated cause additional decrease , the mycorrhizal infection decrease significantly at formalin sterilization in compare with autoclave , the replicated formaline treatment gave the lowest value , this treatment dry soil compare with 1-CFU gm 51 x 10 whichthe number of bacteria and fungi gavefor fungi 510 x 10d for bacteria an 5sterilization treatment which gave 44 x 10 nonrespectively , as well as the mycorrhizal infection percentage of replicated formalin treatment was 20% in compared with 71.60 , 51.66 ,35 and 31.66% for control , Autoclave for one time , Autoclave replicated and formalin fore one time respectively .Also the results showed that sterilization with Autoclave and formalin cause significant decrease in germination percentage, height of soybean plant and the dry weight of shoot and root part in compare with non sterilized treatment , the results indicated that the growth characteristics value of Autoclave treatment was higher than formaline treatment , the formaline replicated treatment gave the lowest value of germination percentage hight plant , shoot and root dry weight .