Abstract
The present study was undertaken to study the effect of stearic acid (SA), a non esterified fatty acid (NEFA) on oocyte development of ewes (Ovis aries). Sheep oocytes were matured in vitro in the presence of different concentration of stearic acid (0 μM, 10 μM, 20 μM, and 30 μM) for 24h. After in vitro maturation, oocytes were in vitro inseminated with cauda epidydimus sperm processed with BO medium with concentration of 2 million sperms/ml and cultured on oviductal cell culture for 8 days. Oocytes were evaluated for cleavage and fertilization rates and after 7–8 days post insemination zygotes were evaluated for moruale /blastocyst stages. The maturation, cleavage and morulae/ blastocyst production rates were significantly lowered in media containing 20 μM stearic acid. Increment of stearic acid to 30 μM in media further reduced the maturation, cleavage and morulae/ blastocyst production. In conclusion, the metabolic stressors NEFA (SA) impaired the maturation, viability, cleavage and embryo production rates at the level 20 μM in ewes.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
