Effect of static magnetic field on pollen-induced allergic airway inflammation in a murine model

Abstract

Event Abstract Back to Event Effect of static magnetic field on pollen-induced allergic airway inflammation in a murine model Aniko Csillag1, Brahma V. Kumar1, Krisztina Szabo1, Kitti Pazmandi1, Eva Rajnavolgyi1, Janos Laszlo2 and Attila Bacsi1* 1 University of Debrecen, Department of Immunology, Hungary 2 University of Debrecen, Department of Computer Science, Hungary It has been demonstrated that pollen NAD(P)H oxidases generate oxidative stress in the airway epithelium and this immediate oxidative insult is crucial for the development of allergen-driven airway inflammation. In this study we aimed to define the effect of inhomogeneous static magnetic field (iSMF) on pollen-induced allergic airway inflammation since several lines of evidence suggest that iSMF is able to trigger biological responses at least partly through free radical reactions. BALB/c mice were sensitized by two intraperitoneal injection of ragweed pollen extract (RWE) and challenged with RWE intranasally. Inflammation was evaluated by determining inflammatory cell accumulation and mucin levels, as well as histological analysis of the airways. iSMF was generated with an apparatus optimized to small experimental animals. We found that iSMF did not affect the sensitization phase of the allergic responses; however, even a single 30-min exposure to iSMF after i.n. RWE challenge was able to reduce the airway inflammation. In addition, prolonged exposure to iSMF after RWE challenge decreased more effectively the severity of inflammation. In animals exposed to iSMF immediately after challenge, RWE induced a lower increase in the total antioxidant capacity of the airways suggesting that effects of iSMF on allergic inflammation were mediated at least partly by modulation of ROS levels. These data indicate that iSMF is able to reduce the inflammation in an in vivo system, despite the fact that it does not interfere with ROS-production of RWE NAD(P)H oxidases directly in cell free conditions. Grants: TAMOP 4.2.2.A-11/1/KONV-2012-0023, Janos Bolyai Fellowship, Bridging Fund 2012 Keywords: Oxidative Stress, static magnetic field, pollen allergy, Allergic Airway Inflammation, mouse model Conference: 15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013. Presentation Type: Abstract Topic: Immune-mediated disease pathogenesis Citation: Csillag A, Kumar BV, Szabo K, Pazmandi K, Rajnavolgyi E, Laszlo J and Bacsi A (2013). Effect of static magnetic field on pollen-induced allergic airway inflammation in a murine model. Front. Immunol. Conference Abstract: 15th International Congress of Immunology (ICI). doi: 10.3389/conf.fimmu.2013.02.00255 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 13 Mar 2013; Published Online: 22 Aug 2013. * Correspondence: Dr. Attila Bacsi, University of Debrecen, Department of Immunology, Debrecen, H-4032, Hungary, bacsi.attila@gmail.com Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Aniko Csillag Brahma V Kumar Krisztina Szabo Kitti Pazmandi Eva Rajnavolgyi Janos Laszlo Attila Bacsi Google Aniko Csillag Brahma V Kumar Krisztina Szabo Kitti Pazmandi Eva Rajnavolgyi Janos Laszlo Attila Bacsi Google Scholar Aniko Csillag Brahma V Kumar Krisztina Szabo Kitti Pazmandi Eva Rajnavolgyi Janos Laszlo Attila Bacsi PubMed Aniko Csillag Brahma V Kumar Krisztina Szabo Kitti Pazmandi Eva Rajnavolgyi Janos Laszlo Attila Bacsi Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.