Effect of static magnetic field on α-amylase activity and enzymatic reaction

Abstract

The effect of magnetic field on α-amylase was studied. Under the experimental conditions, α-amylase solution was treated by 0.15 T, 0.30 T and 0.45 T static magnetic fields for a known period of time, then the activity, kinetic parameters, and the secondary conformation were investigated. The results showed that there was a considerable effect of the magnetic exposure on the α-amylase. The activity was increased by 27%, 34.1%, 37.8% compared with the control. It was also found that both kinetic parameters K m and V m could be decreased due to the increasing magnetic field, K m decreased from 2.20×10−2 to 0.87×10−2, whereas V m decreased from 2.0×10−3 g/min to 1.1×10−3 g/min. At the same time, there were some irregular changes in α-amylase secondary conformation.