Effect of starvation and refeeding on amino acid metabolism in muscle of crab Neohelice granulata previously fed protein- or carbohydrate-rich diets

Abstract

The present study assesses the effects of starvation and refeeding on 1-[14C]-methyl aminoisobutyric acid (14C-MeAIB) uptake, 14C-total lipids, 14CO2 production from 14C-glycine, 14C-protein synthesis from 14C-leucine and Na+–K+-ATPase activity in jaw muscle of Neohelice granulata previously maintained on a carbohydrate-rich (HC) or high-protein (HP) diet. In N. granulata the metabolic adjustments during starvation and refeeding use different pathways according to the composition of the diet previously offered to the crabs. During starvation, 14CO2 production from 14C-glycine, and 14C-protein synthesis from 14C-leucine were reduced in HC-fed crabs. In crabs maintained on the HP or HC diet, 14C-total lipid synthesis increased after 15days of starvation. In crabs fed HP diet, 14C-MeAIB uptake and Na+–K+-ATPase activity decreased in refeeding state. In crabs refeeding HC diet, 14C-MeAIB uptake and 14CO2 production decreased during the refeeding. In contrast, the 14C-protein synthesis increased after 120h of refeeding. In both dietary groups, 14C-total lipid synthesis increased during refeeding. Changes in the carbon amino acid flux between different metabolic pathways in muscle are among the strategies used by this crab to face starvation and refeeding. Protein or carbohydrate levels in the diet administered to this crab modulate the carbon flux between the different metabolic pathways.