Abstract
We followed the phosphorylation of ADP by 32Pi coupled to oxydation of dl-β-hydroxybutyric, succinic and pyruvic acids during incubation of mitochondria with crude staphylococcal α-toxin at 30°C. A heat stable factor caused a complete loss of mitochondrial phosphorylation after a lag period of 1–2 min. This effect could not be substantially reduced either with phospholipids (lecithin, cephalin) or with metopirone.
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