Effect of staphylococcal α-toxin on the phosphorylation of ADP by rat liver mitochondria—I. Inhibition of phosphorylation by a thermo-stable factor

Abstract

We followed the phosphorylation of ADP by 32Pi coupled to oxydation of dl-β-hydroxybutyric, succinic and pyruvic acids during incubation of mitochondria with crude staphylococcal α-toxin at 30°C. A heat stable factor caused a complete loss of mitochondrial phosphorylation after a lag period of 1–2 min. This effect could not be substantially reduced either with phospholipids (lecithin, cephalin) or with metopirone.