Effect of standardized extract of Cosinium fenestratum stem bark on liver and kidney function parameters in streptozotocin-induced diabetic rats

Abstract

ObjectiveTo investigate the effects of standardized dichloromethane (DCM) extract of Cosinium fenestratum (C. fenestratum) stem bark on liver and kidney function parameters in streptozotocin (STZ)-induced diabetic rats. MethodsStandardization of the extract was performed through high performance liquid chromatography (HPLC) using berberine (BE) as marker compound. The standardized C. fenestratum stem bark extract (SCFE) was administered orally at a dose of 100 mg/kg to STZ-induced diabetic rats for 15 d. ResultsThe quantity of BE in the extract was 2.09% w/w. Blood glucose levels, blood urea nitrogen (BUN), alkaline phosphatase (ALP) and creatinine were significantly (P<0.05) elevated in STZ-induced diabetic rats as compared to normal control groups. Treatment of diabetic rats with the extract significantly (P<0.05) reduced, ALP, creatinine, and BUN levels as compared to the diabetic control group. The total white blood cells (WBC) count was reduced in diabetic rats. Treatment of diabetic rats with SCFE significantly (P<0.05) increased the total WBC count as compared to the values of diabetic control rats. ConclusionThe observations of the present study show that extract of C. fenestratum stem bark has hepato-renal protective effect in STZ-induced diabetic rats.