Abstract
The present study aimed to measure the lateral diffusion coefficient of lipids in hepatocyte plasma membranes ( D l ) by the fluorescence recovery after photobleaching (FRAP) technique using N-4-nitrobenzo-2-oxa-1,3-diasolyl phosphatidylethanolamine (NBD-PE) as a probe after spironolactone (Sp) treatment in rats. D l in male 2-month-old Fischer 344 rats was more than five times faster than the protein lateral diffusion coefficient ( D p) previously determined by the authors. A 4-day pretreatment of rats with a daily dose of 20 mg Sp/100 g body weight significantly increased D l by about 10% ( p<0.01). This finding is in contrast with two previous studies reporting a reduction of the rotational mobility of lipids in hepatocyte plasma membranes after Sp pretreatment, but is in parallel with the changes in D p caused by Sp treatment. We suggest that the lateral mobility of proteins and lipids of hepatocyte plasma membranes as determined by the FRAP technique reflects the membrane functions more realistically than does the rotational mobility of lipids, as determined by the fluorescence anisotropy method.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
