Abstract
Enzymes of sucrose metabolism viz. sucrose synthase (SS), sucrose phosphate synthase (SPS) and invertase were studied in the leaves of sugarcane (Saccharum officinarum L.) var. 'Co 7527' under the influence of spentwash as ferti-irrigation with respect to crop age. The activity of SS increased with increasing concentrations of spentwash while higher concentration (1,500 ppm BOD) lowered the activity by 1.42% and 4.16%, respectively, in 9 and 12 months old crop. SPS exhibited higher activity in 9 months old sugarcane with stimulatory effect at 1,000 and 1,500 ppm BOD treatment while invertase showed higher activity in 9 months old crop and declined at 12 months by exhibiting stimulatory effect at 1,000 ppm BOD. The elevation in SPS/invertase ratio at higher concentration of spentwash treatment can be attributed to the contributory factor for maintenance of high sucrose level. The study indicated that use of spentwash up to 1,500 ppm BOD as ferti-irrigation does not affect sucrose metabolism.
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