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Abstract
O UR STUDIES on phospholipids and serum lipids were initiated following the observation that incubation of human serum with an emulsion of fats containing soybean phosphatides caused an increase in the migration velocity of serum lipoproteins. It appeared that the phosphatide used as an emulsifier was the active ingredient. I plan to show that the soybean phosphatide complex produces this “lipoprotein shift” and that the inositol phosphatide fraction is responsible for this phenomenon . ‘ The effect of the intravenous administration of this fraction on serum lipids and lipoproteins will also be demonstrated. An extract of soybean phosphatide was dissolved in normal human serum. Aliquots of the mixture were streaked on filter paper strips for electrophoretic analysis immediately after mixing and after incubation at 37#{176}C. for twentyfour hours. The strips showed a definite lipoprotein shift or increase in the migration velocity of the beta lipoprotein to the alpha. Addition of the soybean phosphatide to hyperlipemic serum produced a similar effect. Whole soybean phosphatide complex consists of approximately equal quantities of lecithin, cephalin and inositol phosphatide, or lipositol. Fractionation with alcohol produces an alcoholsoluble lecithin fraction and an alcohol-insoluble lipositol fraction, both of which contain onethird cephalin. The alcohol-soluble fraction contains two-thirds lecithin and the alcohol-in
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