Effect of Soybean Extract Cream on Matrix Metalloproteinase-1 (MMP-1) and Interleukin-6 (IL-6) Levels (In Vivo Experimental Study on UVB-Induced Balb/c Mice)

Abstract

UV radiation causes skin disorders that start from the formation of reactive oxygen species (ROS) and the synthesis of MMP-1 by dermal fibroblasts which plays a role in skin aging, resulting in an increase in IL-6 levels which correlates with tissue damage and inflammation that occurs. The compounds contained in soybean extract are known to act as anti-inflammatory and antioxidants which can reduce inflammation and reduce the negative impact of UV rays on the skin. This study aims to determine the effect of giving soybean extract cream on MMP-1 levels and IL-6 levels in the skin tissue of bablb/c mice induced by UVB light. This research design was a posttest only control group with a completely randomized design method. The samples studied were 36 female balb/c mice with UVB light induction with a wavelength of 302 nm and an energy of 1 MED 5 times a week for 2 weeks. This research was carried out in five groups, namely the healthy group (K1), negative control group (K2), positive control group (K3), with treatment group 1 (K4) with 10% soybean extract cream and treatment group 2 (K5) with extract cream. soybeans 20%. MMP-1 levels and IL-6 levels were analyzed using ELISA. ELISA analysis showed that there was a significant decrease in the mean MMP-1 concentration between K2 (6488 ± 805) compared to K5 (5396 ± 522) with a p value 0.05. Analysis of IL-6 concentration also showed that there was a significant decrease in the mean IL-6 concentration in K4 (270±50) and k5 (242±54) compared to K2 (661±90) with a p value 0.05. Giving soybean extract cream can reduce MMP-1 and IL-6 levels in female Balb/c mice induced by UVB light.