Abstract
Eight vertebrate and invertebrate hormones were screened for their effect on population growth, mictic female production, and body size of the marine rotifer Brachionus plicatilis. Growth hormone (GH) or human chorionic gonadotropin (HCG) at 0.0025–25 I.U. ml−1 and estradiol-17s (E2), triiodothyronine (T3), 20-hydroxyecdysone (20-HE), 5-hydroxytryptamine (5-HT), gamma-aminobutyric acid (GABA) or juvenile hormone (JH) at 0.05–50 mg 1−1 were added to 5-ml of Nannochloropsis oculata suspension (7 × 106 cells m1−1). From an initial density of 1 individual m1−1, rotifers were cultured with hormones for 48 hours in 22 ppt seawater at 25 °C, in darkness. Rotifers were counted and classified into female types and transferred to a new algal food suspension without hormone every other day until day 8 when body size was measured.
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