Effect of Some Senescence Retardants Pretreatment on Yield of Protoplasts from Spinach Beet (Beta vulgaris) Leaves

Abstract

Summary Spinach beet (Beta vulgaris) leaf tissues were treated with cycloheximide (0.5 mg/ml), penicillin (500 mg/ml), kinetin (0.5 mg/ml) alone or in combination for different periods prior to protoplast isolation and the yield of intact protoplasts, on chlorophyll basis, was found to be maximal after 18 h in cycloheximide pretreated leaves and after 6 h in penicillin and kinetin pretreated leaves. Though cycloheximide effect required long preincubation period, the yield of protoplasts was highest.