Abstract
Summary Vegetative growth and antheridial formation in the male clones of Microdus brasiliensis are affected by light level (intensity), photoperiod, temperature and hydration of the medium. Increase in light level up to 4,500 lux increases the total number of gametophores as well as the number of fertile gametophores per culture. Gametangial induction and vegetative growth increase with increasing photoperiod, responding optimally with continuous illumination. A temperature of 18 °C and a medium with 0.8% agar is optimal for gametangial induction and vegetative growth. With 2.4% and 3.2% agar, vegetative growth is reduced and no antheridia are induced.
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