Effect of some of the natural organic sources on rice tissue culture

Abstract

Objective The main goal of this investigation was to evaluate the effect of glutamine, tryptophan, and casein hydrolysate on callus induction and shoot regeneration in two Egyptian rice cultivars (Sakha104 and Giza178). Materials and methods Different concentrations of tryptophan, glutamine, and casein hydrolysate were investigated separately for the maximum production of callus and shoot regeneration. Results and conclusion Although tryptophan demonstrated a stimulatory effect on callus induction of Sakha104 cultivar, it showed no positive effect on callus initiation of Giza178 genotype and shoot regeneration of both cultivars. Inclusion of glutamine did not enhance either callus induction or shoot regeneration in both cultivars. Supplementation of appropriate amounts of casein hydrolysate resulted in positive response in both callus induction and shoot regeneration. In addition, these responses varied significantly between the two tested cultivars. Irrespective of type and concentration of the natural organic source, Sakha104 proved to have better regeneration capacity than that of Giza178.