Effect of some heavy metals synergism on the longevity and bioaccumulation inside the muscles of frog ridibunda species under the laboratory conditions

Abstract

Rana ridibunda frogs (Amphibians) are examined under laboratory conditions (10-18 C°) of the aquarium water (tap water), oxygen saturation, non-saturation and starvation. Body weight for two groups of frogs were (260-285 gr) and (58-115 gr). Two types of heavy metals effect were examined (single type and mixed types) of Cdcl2 and HgSo4 used. Longevity as LT50% and LT100% were determined by single heavy metals effect and synergism effect for both oxygen saturated and oxygen non-saturated waters experiments were conducted, then the bioaccumulation inside the legs muscles of the frog were examined for the heavy metals poisoning media (2PPT) concentration. Synergism effects were very clear and differences of O2 saturation were giving clear different results.