Effect of some effective protein compounds isolated from Chicory leaves on some blood and biochemical varieties in female rats with anemia creator bleeding

Abstract

This study was designed to isolate some of proteinous compounds from the aqueous extract of Chicory Leaf by cold acetone precipitation. Using gel filtration technique, two compounds (A and B) were isolated from the proteinous precipitate. Then, the effects of aqueous extract and the proteinous precipitate were studied on some blood values and biochemical variables in mice infected with anemia creator bleeding. These extracts were administrated intraperitoneally and their effects were compared with estate ferrosam. After one week of treatment the results indicated that the aqueous extracts and their isolated compounds caused a significant increase in packed cell volume (PCV%), white blood cells (WBC) count and total protein in female mice comparing with control group. The results also indicated that treatment with the aqueous extracts crude and proteinous precipitate at doses of 150, 15 mg/Kg body weight respectively produced a significant increase of glutathione and a significant decrease in uric acid the normal level. The results were also showed that compound A and B at the dose (0.937, 1.05) mg/Kg body weight respectively access level serum iron, hemoglobin blood, PCV% and total protein to the normal level, in addition to similar effect to estate ferrosam effect.