Abstract
Protonema of Bryum pallescens remained bud-free on Nitsch's basal medium (NB). Of the four auxins tried 2,4-dichlorophenoxyacetic acid (2,4-D) and β-naphthoxyacetic acid (NOA) promoted protonemal growth, but α-naphthaleneacetic acid (NAA) and indole-3-acetic acid (IAA) proved inhibitory. Buds failed to appear on media supplemented with 2,4-D, IAA and NAA but a few buds were induced by NOA. Both the antiauxins tested, maleic hydrazide (MH) and 2,3,5-triiodobenzoic acid (TIBA) enhanced protonemal growth. Buds were induced at higher concentrations of MH, whereas no buds were observed at any level of TIBA. Addition of MH (10−4 M) with IAA (10−8 M) led to the formation of a few buds.
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