Abstract
Chromatography is the procedure for analysing a multicomponent mixture of trace, minor or major constituent by separaationinto its individual fractions.For the analytical determination of sildenafil in pharmaceutical dosages forms an expeditious & sensitive RP-HPLC method is described.The mobile phase composition for chromatographing sildenafil was acetonitrile: methanol: water(60:25:15, V/V/V) with a flow rate of 1mL/min using C18 column. The internal standard selected was racecadotril and its eluents were observed at 217nm. The retention time was 4.919 min for the drug. Between 5-200μg/mL the linearity of this method was observed. For analysis of drug in tablets, the depicted method was found to be appropriate. The results of the analysis was evaluated statistically. The method were found to be robust, accurate and economic with a major advantage of column longevity, as buffer was not used throughout the method. Hence the proposed method can be extended for routine analysis.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
