Abstract
The variability of ESMS charge state distributions was investigated for a number of analytes when the ionic strength of their solutions was altered over a wide range. Solution ionic strength was adjusted by adding varying amount of electrolytes (NH 4 OAc, CsCl or (n-C 4 H 9 ) 4 N + Cl - ) to protein solutions of myoglobin and lysozyme. Measured solution QH values were found to be nearly constant over the entire range of CsCl and (n-C 4 H 9 ) 4 N + Cl - addition, and a slight increase in QH was observed upon NH 4 OAc addition. Analyte peak intensities in positive ion ESMS reproducibly decreased as the solution ionic strength increased. However, analyte charge state distributions remained quite constant desite 4 orders of magnitude variation in electrolyte concentration
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