Effect of soluble CD44 molecules on the apoptosis of cultured trabecular meshwork cells from patients with primary open-angle glaucoma

Abstract

Objective To investigate the effect of different concentrations of soluble CD44 molecule (sCD44) in trabecular meshwork cells from patients with primary open angle glaucoma (POAG)on the apoptosis of trabecular meshwork cells,to study the relationship between sCD44 and POAG,and further investigate the pathogcnesis of POAG.Methods Human trabecular meshwork cells were primarily cultured and subcultured.The 3rd passage cells were incubated with different dosages of sCD44 at (1,5,10,25,50ng/ml) final concentration,diluted by DMEM/F12 without serum for 24hours,using CCK-8,fluorescence microscopy and flow cytometry method to study the effect of sCD44 on the apoptosis of cultured trabecular meshwork cells.Results With increasing concentration of sCD44,the inhibition on POAG trabecular meshwork cells were enhanced,by cell counting kit-8(CCK-8),and the experimental group compared with the control group and within experimental group was statistically significan (P ＜ 0.05 ).By fluorescence microscopy showed that the early and late apoptotic cells were increased with the increasing final concentration of sCD44.The experimental group□s apoptosis rate of the different dosages of sCD44 at (1,5,10,25,50ng/ml) final concentration were:10.7283 ± 0.0223,17.3267 ± 0.0250,21.1483 ± 0.0248,25.1267 ± 0.0281,29.9900 ±0.0335,respectively by flow cytometry method,which were higher than the control group of 2.5550±0.0187.The experimental group compared with the control group and within experimental group the difference was statistically significant (P ＜ 0.05).Condusions In a certain range,sCD44 can inhibit the proliferation of trabecular meshwork cells,cause apoptosis,and show a dose dependent manner.The changing of sCD44 levels may be acting on the trabecular meshwork and indirectly involved in the pathogenesis of POAG. Key words: sCD44; Primary open-angle glaucoma; Trabecular meshwork; Cell culture