Abstract

In this study, a mesophilic two-phase anaerobic digestion system was used to treat waste activated sludge at the temperature of 35 ± 1 °C. The internal structure of the anaerobic sequencing batch reactor (methanogenic phase) has been improved by providing a solid–liquid separation plate, and a continuous-flow stirred tank reactor was employed as the acidogenic phase. The results of the study indicate that solid–liquid separation can be achieved in the methanogenic phase, and the low-temperature hydrolysis pretreatment can enhance solid–liquid separation capability. The separation of solids retention time and hydraulic retention time was also achieved in methanogenic phase, and the solids retention time was extended, which effectively improved the anaerobic digestion performance of the system. The organic concentration and total solids concentration in the methanogenic reactor were significantly different in height. The bottom of the reactor formed an area of high solids and low organic precipitation, while the upper part of the reactor formed a clear water area. The suspended solids concentration in the reactor clear water area was maintained at 359 ± 84 to 533 ± 93 mg/L, and the better solid–liquid separation effect was obtained inside the methanogenic phase. The maximum biogas yield was 258 ± 3 mL/g with the maximum volatile solids degradation rates of 59.43% in the system in Stage 3. Considering the efficiency of anaerobic digestion and the operating costs, the operating condition of Stage 4 (the designed hydraulic retention time was 10.67 d, the feed sludge was thermally pretreated, and its volume was 3 L) in this test is the optimal.

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