Effect of Sodium selenite administration on the process of tendon healing in Wistar male rats.

Abstract

Tendon lesions have a great effect on the quality of life and medical spending. Thus is important to investigate the mechanisms responsible for tendon healing and to identify novel treatment options. The aim of the present study was to evaluate the effect of Selenium on the healing processes of injured tendons. A total of 20 Wistar male rats were used and were split into two groups with two different treatment methods. The first group received a normal food administration, while the second group received Na2SeO3. The animals were kept for 28 days. During the eighth day, all animals underwent surgical experimental Achilles tendon lesion and a Kessler-type suture. After three weeks, the animals were sacrificed and the tendon was extracted for histological evaluation in order to do a comparison according to the Movin scale (modified by Bonar). The histological evaluation revealed an even orientation of the collagen fibers in the case of the experimental group (Se) compared with the second group. The Bonar score was 1.62 for the Se group, while the control group had a Bonar score of 1.98. The average number of tenocytes in the Se group was lower which is demonstrated by a lower Bonar score (1.22), compared with the second group (Bonar Score 1.85). In addition, a slightly higher number of tenocytes compared with the intact tendon areas was recorded. In vascularization, a decreased amount of blood vessels in the experimental group (Se) was observed (Bonar Score 1.70), compared with the control group (Bonar score 1.96). The present study demonstrated that Selenium administration on murine models could be beneficial for tendon healing. Further clinical research is required in order for this to be confidently recommended.