Effect of sodium butyrate on the production of serotonin, histamine and glycosaminoglycans by cultured murine mastocytoma cells

Abstract

Effect of sodium butyrate on the cellular serotonin, histamine and glycosaminoglycans (GAGs) contents of mastocytoma p-815 cells was examined. When mastocytoma p-815-4 cells were cultured for 4 days in the presence of butyrate at 2 mM, the optimal concentration for the induction of granulopoiesis in this cell line, the cellular serotonin, histamine and GAGs contents increased markedly: serotonin increased almost 7 times, histamine 140 times and total GAGs 10 times. Chondroitinase ABC-resistant GAGs, heparin and/or heparan sulfate, increased 21 times. These cellular products reached at 3 or 4 days culture their maximal levels which depended on the amount of butyrate added up to 2 mM. The effectiveness of butyrate varied among the cloned cell lines: serotonin and histamine contents did not increase in mastocytoma p-815-6 cells, in which butyrate failed to induce granulopoiesis.