Effect of Smoke Exposure on Chronic Inflammation and P53 Expression in Pelvis Epithelial

Abstract

Many risk factors can cause upper urinary tract carcinoma. Smoking is the most influential risk factor and is associated with the formation of aromatic acids and could induce apoptotic cycle. This research was aimed to examine the effect of cigarete smoke exposure on inflammatory state and p53 expression in renal pelvis epithelial of wistar rats. As many as 25 male rats aged 6-8 weeks were divided into five groups as follows: Control (without treatment); CS-1, CS-2, CS-4, and CS-8 (treated with CS 1x, 2x, 4x, and 8x/day, respectively). Each exposure was done for 15 minutes for 60 days. histopathological changes were evaluated from HE-stained specimens and immunohistochemistry method was applied to measure p53 expressio neutrophile and lymphocyte count in CS-8 was significantly higher as compared to CS-1 (p<0.05) and control (p<0.05). p53 expression was found in the CS-8 (3 out of 5 subjects had positive p53 expression, with a total of 4-7 cells from 10 hpf). Correlation study showed significant correlation between frequency of cigarette smoking exposure and neutrophile count (p=0.000; r=-0.856); lymphocyte count (p=0.000; r=0.985); and p53 expression (p=0.000; 0.072). We concluded that there were significant differences in acute inflammation state, chronic inflammation state and p53 expression among groups. Correlation study showed that frequency of cigarette smoking exposure was positively correlated with lymphocyte count and p53 expression.