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Abstract
The effect of insulin in vivo on the proliferation and cellularity of epididymal adipose tissue of growing rats was investigated. Following the intraperitoneal administration of small amounts of insulin (500 μU/ rat, twice a day), which did not influence the blood sugar level and food intake, it was found that repeated administration of insulin for 48–72 h leads in adipose tissue to an enhanced incorporation of 14C-2-thymidine into DNA and to an increase of the total amount of DNA and RNA in the fat body. The enhanced DNA synthesis in adipose tissue of insulin-treated rats was marked in nuclear DNA and absent in mitochondrial DNA. After fractionation of adipose tissue by collagenase an enhanced DNA synthesis was found in the fraction of adipose and stromavascular cells. Morphological examination of adipose tissue of insulin-treated rats revealed cells in the phase of mitotic division and an increased ratio of fat cells of smaller size. Calculation of the number of cells in the epididymal fat body revealed that, after administration of insulin, the number of adipose and stromavascular cells increased.
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