Abstract
Objective To investigate the effect of siRNA targeting TGF-β inducible early gene(TIEG) on expression of Smad2 in advanced glycation end products(AGEs)-mediated renal tubular epithelial cells.Methods The pshRNA-copGFP-lentivector containing target gene was used as a vector to construct siRNA-TIEG,which was then transfected into normal rat proximal tubular epithelial cells(NRK52E),which were then cultured in RPMI 1640 medium supplemented with AGE-BSA for 24 h and 48 h.The expression of Smad2 mRNA and protein was examined by PCR,Western blotting analysis,respectively.Blank vector group served as control.Results SiRNA-TIEG significantly reduced the expression of TIEG mRNA,Smad2 mRNA and Smad2 protein in normal rat proximal tubular epithelial cells in presence of AGE-BSA compared with those in the control group(P 0.05).Conclusion Silence of TIEG can effectively inhibit the expression of Smad2 mediated by AGEs in NRK52E cells.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: Academic Journal of Second Military Medical University
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
