Abstract
ABSTRACTWe reported a mammalian cell-imaging paradigm to study the cellular response to single-walled carbon nanotubes (SWCNTs). Chinese Hamster Ovarian (CHO) cells were exposed to SWCNTs resuspended in physiologically compatible buffer (phosphate buffered saline, PBS), at concentrations ranging from 0 to 50 μg/mL. Upon exposure, we optically imaged the cells in order to (1) visualize the accumulation SWCNTs in cells in real-time; (2) qualitatively and quantitatively assess the morphological changes associated with cellular stress in the presence of SWCNTs; and (3) serially quantify cell survival with highly sensitive bioluminescence-based imaging. Our results showed that the cell survival obtained from optical imaging agreed with that from CellTiter-Glo (CTG) luminescence viability assay. Acute compromise in the CHO cell’s survival rate was observed under high concentrations of SWCNT exposure. The cellular response as a function of SWCNT concentrations, and exposure time was further investigated.
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