Abstract
Rates of gastric acid and pepsin secretion into the gastric juice were examined in terms of enzymatic activity (measured spectrophotometrically as tyrosine released from hemoglobin substrate) and total gastric enzyme secretion (calculated from secretion of 14C-labelled protein) at various intervals up to 9 hr following introduction into the stomach of one or two glass beads representing approximately 0.62 and 1.26% of the bluegill (Lepomis macrochirus) body weight, respectively. Observations of enzymatic activity and concentration suggested that gastric secretory reserves would have been depleted 6 hr after consuming a meal equalling 1.26% of the fish body weight. Decreased acidity of gastric juice 5 hr after ingesting two glass beads supported this interpretation. The importance of these data to efficiency and growth studies is discussed.
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