EFFECT OF SILVER NANOPARTICLES SYNTHESIZED USING LEAVES EXTRACT OF OLIVE ON HISTOPATHOLOGICAL AND CYTOGENETIC EFFECTS IN ALBINO MICE

E H Al-Taee

doi:10.36103/ijas.v51i5.1155

Abstract

This study was conducted to examine the possible effects of Ag-NPs synthesized using the olive leaf extract on histopathology and cytogenetic effect in mice. A total of thirty albino mice aged two months were divided into five treatment groups as follows: Group 1: served as control was administrated orally with 0.3 mg/kg bw of normal saline; Group 2 and 3 were administrated orally with varying doses of Ag-NPs synthesized on olive leaf extract (10 and 100 mg/kg bw, respectively) for 30 days; Group 4 and 5 were administrated orally with olive leaf crude extract (300 and 1000 mg/kg bw, respectively) for 30 days. At the end of the experimental period, the detection of possible chromosomal aberrations in blood samples and histopathology (liver, spleen, kidney, uterus, and testes) were carried out. Statistically significant differences (P<0.05) were observed for the chromosomal aberrations in all Ag-NPs groups compared to control and to crude olive leaf extract groups. Histopathological study revealed various alterations in internal organs at high doses of Ag-NPs group including inflammatory reaction, blood congestion, degeneration, fibrosis, mononuclear cell lesion, and necrosis. Slight changes were identified at both doses of crude olive extract treated groups. Based on these results, oral administration of Ag-NPs could cause genotoxic and inflammatory responses in mice and this could be representing a risk to both environment and human health.

Highlights

Nanotechnology is recently a field of passionate scientific attention owing to its wide variety of potential uses in industrial, biomedical and therapeutic related products (26)
Thereby, in this study we attempt to examine the possible effects of Ag-NPs on histopathology and cytogenetic in mice and to compare between a crude leaf extract of the olive and Ag-NPs derived using the same olive leaves extract
MATERIALS AND METHODS Preparation of olive leaves extract: Initially, fresh olive leaves were collected from olive trees, and these leaves were cleaned by water and left them for drying in room temperature for 50 days

Summary

INTRODUCTION

Nanotechnology is recently a field of passionate scientific attention owing to its wide variety of potential uses in industrial, biomedical and therapeutic related products (26). Various sources are available for biosynthesis of Ag-NPs such as; ultrasonic, violet radiation and lithography These methods are not considered ecofriendly (25, 27). Plant extract synthesis is considered an alternative method and to be a need of an hour, because it possesses several benefits compared to chemical methods (3, 29, 31) In this regard, olive leaf extract could have several potential health benefits such as protect from heart disease and lowering blood pressure. Synthesis of silver nanoparticles (Ag-NPs) Ag-NPs were synthesized using olive leaves extract according to method proposed by Awaad et al (5). Rays is measured as a function of the diffraction angle 2θ and the specimen's orientation This diffraction pattern is applied to identify the specimen's crystalline phases and to measure its structural properties. The SEM study was carried out by at the University of Tehran, scanning electron microscope (Hitachi FE-SEM model S-4160, Japan) equipped with Energy dispersive X-ray (EDAX); determine the energy of the X-rays microanalysis

Experimental procedure and conditions

RESULTS AND DISCUSSION

G2 G3 G4 G5 LSD