Effect of silver nanoparticles on bactericidal activity and antibiotic sensitivity of <i>Salmonella enteritidis</i>182

Abstract

Studies have been carried out to determine the synergetic effect of the use of combinations of antibacterial substances, including antibiotics, septabic disinfectant and AgNPs. A significant increase in bactericidal activity was revealed in the combination of septabic + AgNPs + nitox and septabic + AgNPs + ceftiofur. Determination of the sensitivity of Salmonella enteritidis 182 to antibacterial drugs showed the presence of resistance to 8 drugs (38.1%), low sensitivity to 7 (33.3%), sensitivity to 6 (28.6%) and the absence of preparations with high sensitivity indicators. After cultivation of S. enteritidis 182 with antibacterial drugs and their combinations, an increase in the number of preparations to which the studied strain was sensitive was found. The presence of sensitivity to 7-10 drugs was revealed, which is 4.7-19.6% higher than in the control indicators. A previously absent high sensitivity to 2-8 antibacterial drugs (9.5-38.0%) was established. The cultivation of S. enteritidis 182 with AgNPs showed the highest increase in antibiotic sensitivity of all the studied combinations of antibacterial agents in the form of the growth inhibition size increase. This suggests a leading role of AgNPs in overcoming antibiotic resistance. Incubation of S. enteritidis 182 after contact with septabic and argovite caused a maximum increase in the diameter of the growth inhibition of the microorganism without decreasing sensitivity to certain types of drugs (with the exception of septabic, where the loss of sensitivity to tylosin was established). When adding various antibiotics to the combination of septabic + argovit, a decrease in the growth inhibition zone or its disappearance were noted. The combined effect of the use of antibacterial drugs together with silver nanoparticles against bacteria with multidrug resistance is described.