Effect of silver nanoparticles on antibiotic resistance of microorganisms in the treatment of endometritis in cows

Abstract

The main etiological factor in endometritis is pathogenic and opportunistic pathogenic microflora entering the uterus during the postpartum period, during estrus, artificial insemination with contaminated sperm. A study was carried out to investigate changes in antibacterial sensitivity of microorganisms during therapy after labor purulent-catarrhal endometritis of cows with a preparation containing silver nanoparticles. To study the role of opportunistic pathogenic microflora in the etiology of postpartum purulent-catarrhal endometritis a clinical study of 150 cows in a farm in the Novosibirsk region during mass calving was carried out. Animals were divided into experimental and control groups according to the analogy principle. The control group received fish oil intramuscularly in a dose of 150 ml with oxytetracycline hydrochloride in a dose of 40 mg/kg of live weight once every 48 hours and uteroton intramuscularly in a dose of 10 ml once every 48 hours. The experimental group received intrauterine injections of argovit 10 % aqueous solution at a dose of 100 ml once every 48 h and uteroton intramuscularly at a dose of 10 ml once every 48 h. It was found that the treatment of postpartum purulent-catarrhal endometritis of cows with argovit decreased the average duration of treatment of the disease by 1.8 times compared to the preparation in the control group. When treating postpartum purulent-catarrhal endometritis of cows with argovit, an increase in antibiotic sensitivity of the isolated microflora to 21 drugs (87.5 %) from 1.2 to 100% was found. In the control group, there was a decrease in antibiotic sensitivity of the isolated microflora to 18 (75 %) preparations from - 1.1 to 28.7 %.