Abstract
The issue of the potential safety of silicon dioxide nanoparticles (SDNPs) remains relevant. In this connection, in order to use the unique capabilities of silicon nanostructures for biomedical purposes, as well as to level their toxic effects, a detailed study of these nanoparticles interaction with cells and tissues in vivo is required.The aim of the research is to reveal morphofunctional changes in a rat's liver after a single parenteral administration of 12 nm silicon dioxide nanoparticles for the period of six months.Material and methods. Using general histological and immunohistochemical methods to study the rats' liver after a single parenteral administration of 1 ml of silicon dioxide nanoparticles at a dose of 7 mg/kg of body weight at a concentration of 2 mg/ml. The sections of the rats' liver were studied by general histological and immunohistochemical methods after injection of 1mL of a SDNPs saline suspension at a concentration of 2 mg/mL (7mg/kg of body weight). Control animals were injected with 1 ml of saline solution. The material was collected in 21 days, 2, 4 and 6 months months after the administration of the SDNPs and it was fixed in 10% neutral formaldehyde.Results. The formation of granulomas in the liver on the 21st day of the experiment and an increase in the number of Kupfer cells were revealed. However, by the 2nd month of the experiment, the number of granulomas significantly decreases compared to the 21st day of the experiment and continues to decrease in subsequent periods. The average size of granulomas decreases during the 2nd month of the experiment and does not change during the subsequent periods of the experiment. After 6 months of the experiment, the morphofunctional state of the liver is characterized by slightly pronounced aseptic inflammation.Conclusion. A single parenteral administration of silicon dioxide nanoparticles causes pronounced aseptic inflammation of the liver, decreasing by the 6th month of the experiment. Connective tissue remodeling in the liver is not observed at all periods of the experiment.
Highlights
Проблема потенциальной безопасности диоксида кремния в виде дисперсных частиц наноразмерной величины сохраняет свою актуальность
The material was collected in 21 days, 2, 4 and 6 months months after the administration of the silicon dioxide nanoparticles (SDNPs) and it was fixed in 10% neutral formaldehyde
By the 2nd month of the experiment, the number of granulomas significantly decreases compared to the 21st day of the experiment and continues to decrease in subsequent periods
Summary
Проблема потенциальной безопасности диоксида кремния в виде дисперсных частиц наноразмерной величины сохраняет свою актуальность. Цель работы – изучение морфофункциональных изменений в печени крысы после однократного парентерального введения наночастиц диоксида кремния размером 12 нм в течение 6 мес. С помощью общегистологических и иммуногистохимических методов исследована печень крыс после однократного парентерального введения 1 мл наночастиц диоксида кремния в дозе 7 мг/кг массы тела в концентрации 2 мг/мл. Показано формирование гранулем в печени на 21-е сут эксперимента и увеличение числа клеток Купфера. Однако к 2-му мес эксперимента число гранулем значимо снижается по сравнению с 21-ми сут эксперимента и продолжает снижаться на последующих сроках. Средний размер гранулем уменьшается на 2-м мес эксперимента и на последующих сроках эксперимента не изменяется. Через 6 мес эксперимента морфофункциональное состояние печени характеризуется незначительно выраженным асептическим воспалением. Однократное парентеральное введение наночастиц диоксида кремния вызывает выраженное асептическое воспаление печени, уменьшающееся к 6-му мес эксперимента. На всех сроках эксперимента не наблюдается соединительнотканного ремоделирования в печени
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
