Abstract
For the first time, the effect mechanocomposite based on biogenic silica and green tea flavonoids (MC) on reduction of oxidative stress in microshoots of strawberry plantlets (cv. “Solnechnaya polyanka”) during in vitro multiplication, shoot elongation and rooting stages were established. Compared with the plantlets cultured in MC-free media, the ones cultured in media supplemented with MC showed increased key antioxidant enzyme activities (superoxide dismutase – SOD, peroxidase – POD, catalase – CAT). SOD activity increased from 1.1 to 1.4 times at 2.5 and 5.0 mg L-1 MC, POD activity – from 1.8 to 3.8 times at 5.0 and 10.0 mg L-1 MC, and CAT activity – from 1.1 to 1.4 times at 5.0 mg L-1 MC depending on the stage of micropropagation. The high antioxidant enzyme activities correlated with the decrease of H2O2 content (up to 1.7 times compared with the control). The revealed changes in the physiological status of strawberry plantlets in the presence of MC contribute to the further successful acclimatization of regenerants to ex vitro conditions. Thus, these results have demonstrated the potential of “green chemistry” for the production of healthy plant material of strawberry cultivars.
Highlights
The world production and consumption of cultivated strawberry (Fragaria × ananassa Duch.) is increasing very fast
A time dependent variation in the H2O2 content was found in the plantlets during in vitro shoot multiplication, shoot elongation and rooting stages (Table 1)
An increased H2O2 content was observed in the plantlets at shoot multiplication and rooting stages
Summary
The world production and consumption of cultivated strawberry (Fragaria × ananassa Duch.) is increasing very fast. According to Faostat, about 9 million tons of fresh strawberry berries were grown and sold globally in 2017 In this regard, in vitro techniques are important tools for obtaining virus, bacteria and fungus-free strawberry basic planting material [1]. To achieve physiological stability of cultivated plant, the culture medium is optimized by the addition of exogenous compounds with antioxidant properties that activate the antioxidant (non- and enzymatic) defense systems in plant tissues to scavenge reactive oxygen species. In this regard, the selection of antioxidant preparations that minimize the oxidative processes in cells is a necessary step in micropropagation protocols
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