Abstract
Levels of mouse epidermal growth factor (mEGF) in mouse plasma were measured by a sensitive two-site enzyme immunoassay (EIA) to study whether sialoadenectomy (removal of submaxillary gland) affects the level of the factor in the circulation. mEGF levels in plasma of sialoadenectomized mice were lower than those of sham-operated (operated without removal of submaxillary gland) mice, but the difference was not remarkable. That is, the submaxillary gland is not the sole source of EGF in the mouse body.Though the reproductive ability of normal male mice and of those sialoadenectomized or sham-operated was also studied, no significant difference in fertility rate of the mated female mice was found among them. Thus the EGF in submaxillary gland seems not to play important roles in male reproductive function of the mice.
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