Effect of ShRNA targeting silencing of VTCN1 gene on nasopharyngeal carcinoma cells

Abstract

Objective:The aim of this study is to investigate the expression of VTCN1 in nasopharyngeal carcinoma cells and targeting silencing of VTCN1 gene by infected with lentiviral vector to inhibit the proliferation and invasion in nasopharyngeal carcinoma cells. Method:The VTCN1 expression level of different nasopharyngeal carcinoma cells was detected by RT-PCR and Western blotting, the cell lines of the most expression level were seleted to conduct the Subsequent experiments; The lentiviral vector of silenced VTCN1 was transfected into HNE2 cells with VTCN1 expression by lipofectamine 2000, and stable cell lines were screened. Then, the silencing efficiency was detect by RT-PCR and Western blotting; The proliferation and invasion abilities of nasopharyngeal carcinoma cells after VTCN1 gene silencing were detected by ckk-8 and Transwell invasion assay, respectively. The phosphorylation levels of JAK/STAT proteins in nasopharyngeal carcinoma cells with VTCN1 gene silencing were detected by Western blotting. Result:RT-PCR and Western blotting detected that stable transfection of VTCN1ShRNA into HNE2 cells resulted significantly declined expression of VTCN1 (P<0.05); The proliferation and invasion abilities of HNE2 cells were significantly decreased (P<0.05) and phosphorylation level of the JAK, STAT proteins were significantly decreased (P<0.05). Conclusion:VTCN1 ShRNA can effectively silence the expression of VTCN1, and significantly inhibits the proliferation and invasion of HNE2 cells. It may be related to down regulation of protein activity in JAK/STAT signaling pathway..