Abstract
Objective To study the effect of shock waves(SW) on in vitro differentiation of bone marrow stromal cells (hBMSCs) into osteoplasts and determine at which intensity SW can best promote the differentiation. Methods The BMSCs were derived from the bone marrow pool which had been collected from healthy human volunteers. The cells were isolated and cultured in vitro by density centrifugal and an-choring screening. The cells were identified by immunohistochemistry and induced by SW at different inten-sities. The differentiation of BMSCs into osteoplasts was evaluated by alkaline phosphatase Ca-Co staining,calcium node formation and Collagen type Ⅰ immunohistochemistry 40 days later. An optimal intensity of SW was selected to treat BMSCs. RT-PCR was used to evaluate the expressions of c-fos and c-jun. Results The best SW intensity treating BMSCs was 8.5 Kv(120), with shock waves at (0. 230 +0. 015) Mj/mm2.Osteogenesis was significantly clear in the SW groups than in the control ( P ＜ 0. 01 ). Upon the impact of SW at the best intensity, the expressions of c-fos and c-jun grew higher than in the control and reached the peak after 45 minutes. Conclusions SW can promote BMSCs to differentiate into osteoplasts. The optimal intensity of SW treating BMSCs might be 8.5 Kv(120). Key words: Shocwav(SW) ; BMSCs; Osteogenesis; Differentiation
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