Effect of Shenxiong-Huayu capsule preconditioning on cell apoptosis and the expression of C-fos and C-jun in the hippocampal CA1 area of rats with cerebral ischemia reperfusion injury

Abstract

[Abs Objective To observe the effect of Shenxiong-Huayu Capsule preconditioning on cell apoptosis and the expression of c-fos, c-jun in the hippocampal CA1 area of rats with acute cerebral ischemia reperfusion injury. Methods SD rats were divided into 3 groups by completely randomized method： sham operation group（n = 6）, ischemia reperfusion group （model group）, and Shenxiong-Huayu Capsule preconditioning group （preconditioning group）. The last two groups were divided randomly into 6 h, 24 h, 48 h and 72 h reperfusion subgroups （each n=6）. Intragastric administration for 7 days, once a day. Middle cerebral artery occlusion and reperfushion model was made by an intralurninal filament method. Cell apoptosis was detected by TUNEL method, the expression of c-fos, c-jun was observed by immunohistochemistry. Results ①The number of apoptosic cells at 6, 24, 48, 72 h in the hippocampal CA1 area of rats was respectively （11.17± 3.71, 39.83±5.67, 48.33 ±5.32 and 22.17±3.71） single/High power field in the model group, and was respectively （7.83±2.04, 15.00±3.58, 29.50±6.89 and 10.17 ±2.32） single/High power field in Shenxiong-Huayu Capsule preconditioning group. Compared with the model group, the number of apoptosic cells at each time point in thehippocampal CA1 area of rats was decreased in Shenxiong-Huayu Capsule preconditioning group （P〈 0.05 or P〈0.01 ） .②The number of c-los at 6、24、48、72 h in the hippocampal CA1 area of rats was respectively （14.50 ± 3.45,33.67 ± 1.63,42.33 ± 3.32 and 32.00 ± 2.90） single/High power field in the model group, and was respectively （10.17 ± 2.93, 21.50 ± 2.43, 30.83 ± 3.76 and 25.17 ± 5.27） single/High power field in Shenxiong-Huayu Capsule preconditioning group.The number of c-jun at 6, 24, 48, 72 h in the hippocampal CA1 area of rats was respectively （15.50±4.19, 22.83±5.64, 33.10±4.19 and 14.67±3.08） single/High power field in the model group, and was respectively （9.67 ± 3.63, 15.67 ± 2.73, 21.26 ± 3.63 and 9.33 ± 3.61） single/High power field in Shenxiong-Huayu Capsule preconditioning group.Compared with the model group, the expression of c-los, c-jun at each time point in the hippocampal CAI area of rats was decreased in Shenxiong-Huayu Capsule preconditioning group （P〈0.05 or P〈0.01 ） . Conclusion Shenxiong-Huayu capsule can inhibit apoptosis by restraining the expression of c-fos, c-jun and relive cerebral ischemia reperfusion injury in rats. Key words: Cerebral ischemia reperfusion injury; Cell apoptosis; c-los; c-jun; Shenxiong-Huayu capsule