Effect of Shenmai injection on vascular endothelium in rats with focal cerebral ischemia reperfusion

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Objective To observe the effect of Shenmai injection on vascular endothelial function after cerebral ischemia reperfusion in rats. Methods 30 rats were randomly divided into a sham operation group, a model group and a Shenmai group, with 10 in each. Except the sham operation group, animal models of cerebral ischemia reperfusion injury were established by the right internal carotid artery occlusion of middle cerebral artery. Then shenmai injection was given to the Shenmai group, 2 ml/100 g Sodium Chloride was given to the other groups, once per day, for successive 7 days. Endothelin-1 (ET-1), nitric oxide (NO), nitric oxide synthase (NOS), serum high sensitivity C-reactive protein (hs-CRP), and homocysteine (Hcy) were tested. Results In Shenmai group, ET-1(126.10 ± 6.07 ng/ml vs. 105.41 ± 0.09 ng/ml), hs-CRP (15.93 ± 2.79 mg/L vs. 9.33 ± 1.32 mg/L) and Hcy (11.01 ± 0.98 μmol/L vs. 8.13 ± 0.46 μmol/L) were decreased after the treatment, while NOS (168.87 ± 9.25 U/ml vs. 242.25 ± 10.36 U/ml) and NO (84.03 ± 6.02 vs. 102.42 ± 5.05) were increased after the treatment with statistical difference (P＜0.05). Conclusion Shenmai injection can improve NOS activity and promote the synthesis of NO, decrease the level of plasma ET-1, hs-CRP and Hcy, reduce endothelial inflammatory exudation, and prevent vascular endothelial dysfunction caused by cerebral ischemia reperfusion injury. Key words: Shenmai injection; Focal cerebral ischemia; Vascular endothelial