Effect of sevoflurane-containing HTK solution on electrophysiological stability of rat donor heart during reperfusion

Abstract

Objective To evaluate the effect of sevoflurane-containing HTK solution on electrophysiological stability of rat donor heart during reperfusion. Methods Male Sprague-Dawley rats, weighing 280-320 g, aged 3 months, in which a Langendorff-perfused isolated rat donor heart model was established, were used in this study.Sixteen donor hearts were obtained and divided into 2 groups (n=8 each) using a random number table method: control group (group C) and sevoflurane group (group S). HTK solution was used as preservation solution in group C. HTK solution containing 2.5% sevoflurane was used as preservation solution in group S. Hearts were stored for 6 h in the corresponding preservation solution at 4 ℃ and then reperfused, and the perfusion temperature was gradually restored to 37 ℃ in two groups.The time of spontaneous recovery of heart beat and development of arrhythmia were recorded.Heart rate, monophasic action potential (MAP) duration at 50% and 90% repolarization (MAPD50, MAPD90), MAP amplitude and maximum velocity and development of early after-depolarization and delayed after-depolarization were recorded at 30 and 45 min of reperfusion. Results Compared with group C, the time of spontaneous recovery of heart beat was significantly shortened, the incidence of ventricular fibrillation and arrhythmia score were decreased, the ventricular fibrillation interval was shortened, and MAPD50 and MAPD90 of epicardium and endocardium were shortened at 30 and 45 min of reperfusion (P 0.05). Early after-depolarization and delayed after-depolarization were not found in two groups. Conclusion Sevoflurane-containing HTK solution can maintain electrophysiological stability of rat donor heart during reperfusion and decrease the occurrence of arrhythmia. Key words: Anesthetics, inhalation; Heart transplantation; Cardiac electrophysiology