Effect of Semelil, an Herbal Selenium-Based Medicine, on New Bone Formation in Calvarium of Rabbits.

Amir Alireza Rasouli-Ghahroudi,Mohammad Abdollahi,Siamak Yaghobee,Fatemeh Mashhadiabbas ,Amir Reza Rokn

doi:10.1155/2018/2860367

Abstract

Background This study aims to analyze the effect of Semelil, an herbal selenium-based medicine, on osteogenesis in rabbit calvarium defects. Methods Four identical bony defects (8 mm) were created in the calvarium of 16 New Zealand male rabbits and filled randomly with xenogenic bone substitute material (Bio-Oss®) and semelil herbal drug (ANGIPARS™). One site was filled with Bio-Oss (B); the second site was treated with ANGIPARS (A); the third site was treated with ANGIPARS + Bio-Oss (AB); and the fourth site was left as untreated control (C) and defects were left unfilled. Rabbits were randomly divided into two groups (n = 8) and sacrificed at four and eight weeks. Percentage of new bone formation, type of the newly formed bone, percentage of the remaining xenograft biomaterial, and foreign body reaction (FBR) were evaluated via histological and histomorphometric analyses. Results The percentage of new bone formation was significantly different among four groups. The highest effect was observed in AB, followed by A, B, and C groups, respectively. The difference in the mean percentage of new bone formation between four and eight weeks was significant for all four groups (P < 0.001). Regarding bone formation, the interaction effect of A and B was significant at four (P < 0.001) and eight weeks (P = 0.002). ANGIPARS alone and in presence of Bio-Oss enhanced new bone formation at both four and eight weeks (P < 0.001). The mean amount of new bone formation was significantly different at four and eight weeks in groups C (P = 0.008), A (P < 0.001), B (P < 0.001), and AB (P = 0.003). FBR was not observed in any group. Conclusion Semelil may be useful as an adjunct to conventional osteoconductive materials in order to enhance osteogenesis.

Highlights

Bone graft materials have extensive clinical applications in medicine and dentistry [1]
Simultaneous presence of the three following requirements is necessary to achieve osteogenesis: (A) presence of osteoblasts or cells with the potential for differentiation into bone forming cells; (B) presence of osteoinductive stimuli to initiate the differentiation of mesenchymal cells to osteoblasts; and BioMed Research International (C) presence of an osteoconductive environment to form a scaffold for growth and proliferation of preosteoblastic cells and their differentiation into osteoblasts for new bone formation
The foreign body reaction (FBR) was not observed in any specimen

Summary

Introduction

Bone graft materials have extensive clinical applications in medicine and dentistry [1]. Autogenous bone is often referred as the gold standard for regenerative and reconstructive procedures due to its optimal biological properties. The biological mechanisms involved in new bone formation at reconstructed sites include osteoinduction, osteoconduction, and osteogenesis [4, 5]. Four identical bony defects (8 mm) were created in the calvarium of 16 New Zealand male rabbits and filled randomly with xenogenic bone substitute material (Bio-Oss5) and semelil herbal drug (ANGIPARS6). The difference in the mean percentage of new bone formation between four and eight weeks was significant for all four groups (P < 0.001). The mean amount of new bone formation was significantly different at four and eight weeks in groups C (P = 0.008), A (P < 0.001), B (P < 0.001), and AB (P = 0.003). Semelil may be useful as an adjunct to conventional osteoconductive materials in order to enhance osteogenesis

Objectives

Methods

Results

Conclusion