Effect of self-secreted interleukin-6 of proliferation and cell cycle of liver cancer cell line HepG2

Abstract

Objective To study the effect of self-secreted interleukin-6 (IL-6) of proliferation and cell cycle of liver cancer cell line HepG2.Methods The interleukin-6 was made to be silence by using RNA interference technology,and then the control groups were established accordindy by using small interfering RNA (siRNA) with insignificant order,liposome only and no transfection and transfected IL-6-RNA.Detect the proliferation and cell cycle by using methyl thiazol tetrazolium (MTT) and flow cytometry technology.Results The expression of IL-6 mRNA were 104.00 ± 10.71,309.00 ± 15.19,162.50 ±9.85,26.75 ±7.54; The cell proliferation rate were 100.00％,160.72％,130.39％,55.54％ by using MTT; the cell proportion in different cell circles was detected by using Flow cytometry technology in different teams as follows:G0/G1 phase account for (68.09 ±0.98)％,(63.28 ±0.92)％,(67.01 ±0.93)％,(73.43±1.16)％; S phase account for (23.04 ±0.99)％,(32.32 ±0.83)％,(25.11 ±1.24)％,(20.34 ±0.20)％; S ±G2/M phase account for (31.91 ±0.98)％,(36.72 ±0.92)％,(32.99 ± 0.93) ％,(26.57 ± 1.16) ％.Conclusion It can supress the expression of IL-6 mRNA in liver cancer cell HepG2 by using RNAi interference technique; it can supress the proliferation and extend the cell circle of liver cancer cell HepG2 when IL-6was silence; its proliferation was promoted and cell circle was shorten when it secreted higher interleukin-6 in liver cancer cell line HepG2. Key words: Interleukin-6 ; Cell proliferation ; Cell circle